1. Background
  2. Data Collection Methods
  3. Data Quality Control
  4. Summary

Key Concepts for Measurement

Fluorometer Calculated Endpoints - Maximum efficiency of PSII

(Maxwell and Johnson, 2000)

(Fm0-F0)/Fm0 = Fv/Fm0 = ΦPSII/qP

This is a measure of the quantum efficiency of PSII if all reaction centers are open

Variables Measured Directly

  1. F0- yield of fluorescence in the absence of photosynthetic light
  2. Fm0 - maximum fluorescence; obtained in the absence of photochemical quenching