GC/MS Calibration Curve
Chromatography Analysis
Operating conditions
- Instrument settings (e.g., temperature, mobile phase flow rate) for an analysis should be described.
- Sample blanks should be run periodically to ensure that no residual analyte remains from the previous sample.
- Instrument calibration with known concentrations of an analyte provides a scale for interpreting results (see figure). Calibration concentrations should span the expected range of analyte concentrations so that sample measurements fall within a linear range of the calibration curve. This should be done on a regular basis, for example:
- At the start of analysis each day if the same type of analyte is being quantified repeatedly.
- After a column is changed.
- When a new analyte is being quantified.